Retrovirus in salivary glands from patients with Sjögren's syndrome.
Identifieur interne : 003D27 ( Main/Exploration ); précédent : 003D26; suivant : 003D28Retrovirus in salivary glands from patients with Sjögren's syndrome.
Auteurs : S. Yamano [Japon] ; J N Renard [Japon] ; F. Mizuno [Japon] ; Y. Narita [Japon] ; Y. Uchida [Japon] ; H. Higashiyama [Japon] ; H. Sakurai [Japon] ; I. Saito [Japon]Source :
- Journal of Clinical Pathology [ 0021-9746 ] ; 1997-03.
English descriptors
- Teeft :
- Acinar epithelial cells, Activity assay, Aids virus, Arthritis rheum, Assay, Cell line, Clin, Clin microbiol, Control subjects, Core proteins, Divalent cation, Epithelial, Epithelial cells, Gland, Gland biopsy specimens, Gland sections, Gland tissues, Human immunodeficiency virus, Human immunodeficiency virus type, Human intracisternal, Immune response, Immunodeficiency, Immunohistochemical assay, Labial, Lymphoblastoid cells cocultured, Lymphotropic virus type, Mers, Monoclonal, Monoclonal antibody, Mononuclear cells, Normal sera, Peripheral blood, Plasma membrane, Polymerase, Polymerase activity, Polymerase chain reaction, Positive control, Primary syndrome, Proc natl acad, Reaction mixture, Retroviral, Retroviral particles, Retroviral proteins, Retrovirus, Salivary, Salivary gland, Salivary gland epithelium, Salivary gland specimens, Salivary gland tissues, Salivary glands, Serum antibodies, Sicca symptoms, Syndrome, Syndrome patients, Thin sections, Transmission electron microscopy, Unknown retrovirus, Western blot, Western blot analysis.
Abstract
AIMS: To investigate the possibility of an immune response to retroviral antigens or of detecting retrovirus in Sjögren's syndrome. METHODS: Retroviruses were sought in labial salivary glands and peripheral blood mononuclear cells from patients with Sjögren's syndrome by immunoblotting assay, immunohistochemical assay, polymerase chain reaction (PCR), reverse transcriptase (RT) activity assay, and transmission electron microscopy. RESULTS: Sera from five of 15 patients with Sjögren's syndrome (33%) reacted against p24 group specific antigen (gag) of human immunodeficiency virus (HIV). Labial salivary gland biopsy specimens from seven of the 15 patients with Sjögren's syndrome (47%) contained an epithelial cytoplasmic protein reactive with a monoclonal antibody to p24 of HIV. PCR was performed to detect HIV and human T lymphotropic virus type I (HTLV-I) genes from salivary gland tissues and peripheral blood mononuclear cells from patients with Sjögren's syndrome. Mn2+ dependent, Mg2+ independent RT activity was detected in the salivary gland tissues in three of 10 patients. A-type-like retroviral particles were observed in epithelial cells of salivary glands by transmission electron microscopy. Target genes for HIV and HTLV-I were not found in any of the salivary gland tissues or peripheral blood mononuclear cells from Sjögren's syndrome patients. CONCLUSIONS: The data suggest the presence of an unknown retrovirus similar to HIV in the salivary gland which might be involved in the pathogenesis of a subpopulation in Sjögren's syndrome.
Url:
DOI: 10.1136/jcp.50.3.223
Affiliations:
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Yamano</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author><author><name sortKey="Renard, J N" sort="Renard, J N" uniqKey="Renard J" first="J N" last="Renard">J N Renard</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author><author><name sortKey="Mizuno, F" sort="Mizuno, F" uniqKey="Mizuno F" first="F" last="Mizuno">F. Mizuno</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author><author><name sortKey="Narita, Y" sort="Narita, Y" uniqKey="Narita Y" first="Y" last="Narita">Y. Narita</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author><author><name sortKey="Uchida, Y" sort="Uchida, Y" uniqKey="Uchida Y" first="Y" last="Uchida">Y. Uchida</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author><author><name sortKey="Higashiyama, H" sort="Higashiyama, H" uniqKey="Higashiyama H" first="H" last="Higashiyama">H. Higashiyama</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author><author><name sortKey="Sakurai, H" sort="Sakurai, H" uniqKey="Sakurai H" first="H" last="Sakurai">H. Sakurai</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author><author><name sortKey="Saito, I" sort="Saito, I" uniqKey="Saito I" first="I" last="Saito">I. Saito</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Medical College, Department of Oral Surgery</wicri:regionArea><wicri:noRegion>Department of Oral Surgery</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Clinical Pathology</title><title level="j" type="abbrev">J Clin Pathol</title><idno type="ISSN">0021-9746</idno><idno type="eISSN">1472-4146</idno><imprint><publisher>BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><date type="published" when="1997-03">1997-03</date><biblScope unit="volume">50</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="223">223</biblScope></imprint><idno type="ISSN">0021-9746</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9746</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acinar epithelial cells</term><term>Activity assay</term><term>Aids virus</term><term>Arthritis rheum</term><term>Assay</term><term>Cell line</term><term>Clin</term><term>Clin microbiol</term><term>Control subjects</term><term>Core proteins</term><term>Divalent cation</term><term>Epithelial</term><term>Epithelial cells</term><term>Gland</term><term>Gland biopsy specimens</term><term>Gland sections</term><term>Gland tissues</term><term>Human immunodeficiency virus</term><term>Human immunodeficiency virus type</term><term>Human intracisternal</term><term>Immune response</term><term>Immunodeficiency</term><term>Immunohistochemical assay</term><term>Labial</term><term>Lymphoblastoid cells cocultured</term><term>Lymphotropic virus type</term><term>Mers</term><term>Monoclonal</term><term>Monoclonal antibody</term><term>Mononuclear cells</term><term>Normal sera</term><term>Peripheral blood</term><term>Plasma membrane</term><term>Polymerase</term><term>Polymerase activity</term><term>Polymerase chain reaction</term><term>Positive control</term><term>Primary syndrome</term><term>Proc natl acad</term><term>Reaction mixture</term><term>Retroviral</term><term>Retroviral particles</term><term>Retroviral proteins</term><term>Retrovirus</term><term>Salivary</term><term>Salivary gland</term><term>Salivary gland epithelium</term><term>Salivary gland specimens</term><term>Salivary gland tissues</term><term>Salivary glands</term><term>Serum antibodies</term><term>Sicca symptoms</term><term>Syndrome</term><term>Syndrome patients</term><term>Thin sections</term><term>Transmission electron microscopy</term><term>Unknown retrovirus</term><term>Western blot</term><term>Western blot analysis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">AIMS: To investigate the possibility of an immune response to retroviral antigens or of detecting retrovirus in Sjögren's syndrome. METHODS: Retroviruses were sought in labial salivary glands and peripheral blood mononuclear cells from patients with Sjögren's syndrome by immunoblotting assay, immunohistochemical assay, polymerase chain reaction (PCR), reverse transcriptase (RT) activity assay, and transmission electron microscopy. RESULTS: Sera from five of 15 patients with Sjögren's syndrome (33%) reacted against p24 group specific antigen (gag) of human immunodeficiency virus (HIV). Labial salivary gland biopsy specimens from seven of the 15 patients with Sjögren's syndrome (47%) contained an epithelial cytoplasmic protein reactive with a monoclonal antibody to p24 of HIV. PCR was performed to detect HIV and human T lymphotropic virus type I (HTLV-I) genes from salivary gland tissues and peripheral blood mononuclear cells from patients with Sjögren's syndrome. Mn2+ dependent, Mg2+ independent RT activity was detected in the salivary gland tissues in three of 10 patients. A-type-like retroviral particles were observed in epithelial cells of salivary glands by transmission electron microscopy. Target genes for HIV and HTLV-I were not found in any of the salivary gland tissues or peripheral blood mononuclear cells from Sjögren's syndrome patients. CONCLUSIONS: The data suggest the presence of an unknown retrovirus similar to HIV in the salivary gland which might be involved in the pathogenesis of a subpopulation in Sjögren's syndrome.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Yamano, S" sort="Yamano, S" uniqKey="Yamano S" first="S" last="Yamano">S. Yamano</name></noRegion><name sortKey="Higashiyama, H" sort="Higashiyama, H" uniqKey="Higashiyama H" first="H" last="Higashiyama">H. Higashiyama</name><name sortKey="Mizuno, F" sort="Mizuno, F" uniqKey="Mizuno F" first="F" last="Mizuno">F. Mizuno</name><name sortKey="Narita, Y" sort="Narita, Y" uniqKey="Narita Y" first="Y" last="Narita">Y. Narita</name><name sortKey="Renard, J N" sort="Renard, J N" uniqKey="Renard J" first="J N" last="Renard">J N Renard</name><name sortKey="Saito, I" sort="Saito, I" uniqKey="Saito I" first="I" last="Saito">I. Saito</name><name sortKey="Sakurai, H" sort="Sakurai, H" uniqKey="Sakurai H" first="H" last="Sakurai">H. Sakurai</name><name sortKey="Uchida, Y" sort="Uchida, Y" uniqKey="Uchida Y" first="Y" last="Uchida">Y. Uchida</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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